Providing quality product to the Healing Hands.
GERMAN Manufacturer of HPLC COLUMNS
40 years exprince in the Chromotography
ReproSil SEC
| ReproSil SEC – for Size Exclusion |
|---|
| Reprosil SEC phases are designed for the Gel Filtration Chromatography (GFC) of proteins, peptides and other org. polymers in aqueous systems. In GFC elution order is a function of molecular weight, the largest molecules eluting first and the smallest last. The Silica-based Reprosil SEC packings are available with 3 different pore sizes: |
References of ReproSil SEC
| SEC | |||
|---|---|---|---|
| Packing: | Pore size: | MW of Proteins: | MW of Linear Molecules: |
| - | - | - | (denaturated proteins, peptides, other org. Polymers) |
| Reprosil 50 SEC | 50 A° | 8 000 – 30 000 D | 500 – 10 000 D |
| Reprosil 200 SEC | 200 A° | 10 000 – 500 000 D | 2 000 – 70 000 D |
| Reprosil SEC 4000 | 450 A° | 20 000 – 5 000 000 D | 4 000 – 500 000 D |
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Column: ReproSil-Pur 50 SEC, 5µm (300 x 8 mm) Flow: 0,5 ml/min. Eluent: 50 mM KH2PO4+100mM KCL, pH6,5 |
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Peptide Sample:
1= MW: 3894 D
2= MW: 1593 D
3= MW: 826D
Reprosil 200 SEC, 5 µm (300 x 8 mm)
Flow: 1 ml / min
Eluent: 50 mM KH2PO4+ 150 mM KCL, pH 7.0
Sample :
1 =Thyroglobulin (MW – 670 KD)
2 = Ovalbumin (MW – 44 KD)
3 = Myoglobulin (MW – 17 KD
4 = Phenylalanine Reprosil SEC 4000, 5 µm
A.) 250 x 4.6 mm, Flow: 1 ml / min
B.) 750 x 4.6 mm, Flow: 0,33 ml / min
Eluent: 50 mM KH2PO4+ 150 mM KCL, pH 7.0
Sample:
1= Thyroglobulin (MW – 670 KD)
2= Ovalbumin (MW – 44 KD)
3= Phenylalanine
Typical features for ReproSil 200 SEC, 5 µm (Mat..No.: R25.sec.)
pH-Stability: 2 – 7.5
Pore size: 200 A°
Pore volume: 1.2 ml/g
Capacity: about 10 – 500 µg, depending on column-dimension and protein
Max. pressure: about 200 bars
Dimension: 300 x 8 mm, Eluent: (for all shown separations) 50 mM K-phosphate-buffer, pH=7 + 150 mM KCL, Flow: 1 ml/min., Max.Pressure: 200 bars
GPC
Polymeric phases for organic GPC: Typical dimensions: 300 x 8 mm or 600 x 8 mm iD. Other dimensions on request. for non aquous GPCDaltons
ReproGel PS GPC, 5 µm, 50 A°< 1 000rg0.050. ReproGel PS GPC, 5 µm, 100 A°> 3000rg0.100. ReproGel PS GPC, 5 µm, 500 A°> 20 000rg0.500. ReproGel PS GPC, 5 µm, 1000 A°1 000 – 40 000rg0.103. ReproGel PS GPC, 5 µm,10 000 A°4 000 – 500 000rg0.104. ReproGel PS GPC, 5 µm, 100 000 A°10 000 – 2 000 000rg0.105. ReproGel PS GPC, 5 µm, 1 000 000 A°200 000 – 10 000 000rg0.106. ReproGel PS GPC, 5 µm, linear1 000 – 1 000 000rg0. lin. Available particle sizes: 3, 5, 10 µm
